How to use photoshop to measure the amount of shades of Brown in a given area (Immunohistochemistry)
I try to use photoshop measure intensity area of brownish (some variance) in a part of the image of a mouse knee joint. I have stained a particular marker in these samples and hoped I could quantify because she is extracellular and discreet
Thank you!
Scott, I believe that I came up with a way for you to make selections within your settings and have prepared an example to demonstrate the method. Much depends on whether this technique, using your spot color and a specimen, will work. If so, you can measure the number of pixels in the selection following the instructions I provided previously. Here goes:
1. This sample represents the darker brown spot.
2 build a scale of gray similar to this (I can provide one shown here)
3 Edition > fill with your Brown sample more dark and set its blend Mode to color. (When I used the sample in step 1, this was the result.)
4 attach it to your specimen as shown image file here. (I have not colorized that half of the test image to demonstrate the next step only select the sample Brown even if the black & white area has the same tone value.)
5. with the magic wand, a tolerance of zero and contiguous unchecked value, when you click on a box in the balance its corresponding value will be selected in the image. Here is an example. The histogram can then be used for a number of pixels from all selected areas. Repeat for each sample in your settings.
I hope it does the job for you.
[(Edit: quand vous arrivez àles valeurs plus sombres, vous pouvez constater qu'augmenter la tolérance contribue à la couverture de l'écart entre valeurs.)]
Tags: Photoshop
Similar Questions
-
How to use Labview for measuring the reflectance at different wavelengths automatically
Hello!
I'm using labview to communicate with MS257 (a monochromator). If I do not use Labview, I have to use the hand controller (set a wavelength from 300 to 1100 under a grating (a total of four grids).) It takes a lot of time. So I want to use labview to realize the automation. But I don't know how to do, I still can put only a wavelength as a command for MS257, not making any difference.
Does anyone know how to deal with this?
Thank you for your time.
So where are you stuck? It is a fairly common task. Inside of a loop, you can increment the "xxx" and build a table of responses. Look at the Format function in the chain and a knot of shift register/feedback.
-
How to use photoshop to produce lines of edge weighted, similar to lightroom masking slider
How to use photoshop to mimic the effect of Visual detection of contours, as shown in the Lightroom interface holding the key alt and pulling the "masking" slider located under the options of sharpness?
Here is an example of what I want to do:
Start with this image:
And you end up with this:
I was able to do this by taking a screenshot of my window of lightroom now alt and drag the slider "masking" where I wanted to, but I would like to be able to reproduce this effect in full resolution of the image and without having to use such inelegant solution. I asked my local photography group and, while a lot of people stressed the fact that it is extremely easy to use "curves" to do this, I spent hours looking for and experimenting with the curves and same dialog box never approached to reproduce the effect.
Here's a quick example:
-
How to use photoshop with the same account on more than one laptop?
How to use photoshop with the same account on more than one laptop?
a single user license entitles you to two facilities simultaneous/activations. so, what's the problem?
-
I bought creative cloud but phoshop saying that my purchase was expired I do not know how to use photoshop
Please refer to Cloud Creative applications back in test mode after an update until 2015 for CC
Also, make sure you use the right adobe ID to log in.
Hope this will help you.
Kind regards
Hervé Khare
-
How to use transfer windows for xp to xp. When I plug usb computers are delivered to the top with the same screen and when I try to use the transfer to the new computer, it just said information gathering and sits for hours
Hello, welcome.
The process is automatic and the maximum transfer rate should be about 20 GB/HR. If the process crashes during that long of a time, it's probably because of something internal rather something you're doing wrong.For reference purposes, I included this link. It goes into the details of the migration from XP to Vista (and the basic steps you follow). The instructions are for migrating to Vista via the network, but you can easily follow the steps and choose a different option for your specific installation.http://www.windowsnetworking.com/articles_tutorials/migrating-Vista-using-Windows-easy-transfer.htmlFor both machines, I recommend you to prepare for the transfer using the following procedure:1. click on START > run2 type "msconfig" (without the quotes) and press enter3. go to the "Startup" tab and uncheck all the entries displayed4. click on 'Apply' at the bottom rightDO NOT RESTART YOUR COMPUTER AGAIN5. click on START > run6. Type "cmd" (without the quotes). Right-click on the result at the top of the menu START and select 'run as administrator '.7. type chkdsk /r and press enter8 allow the system to attempt disassembly of the volume9. allow the system to plan restarting10. restart your computer11 let checkdisk analyze the file system on your hard drive12. Once completed, perform the same steps on the other machine13. Once completed, repeat the transfer
Let us know what happensThank you!Ryan Thieman
Microsoft Answers Support Engineer
Visit our Microsoft answers feedback Forum and let us know what you think. -
Hello, the following problem, I use a PC, can not use Photoshop, you get the following error message on the computer-Adobe Photoshop CC 2015 has stopped working " ", "the program '. '. have uninstalled et installed a number sometimes but the CC2015 does not start anyway. Soren
Please see:
Re: adobe photoshop cc 2014 has stopped working in windows 8
Re: Photoshop CC 2015 has stopped working
https://www.YouTube.com/watch?v=0OUdIZT7C2E
I hope this helps.
Concerning
Megha Rawat
-
Good night, I'm just starting bussines Design, I'm not rich enough to buy a license of photoshop, but I am loved to use photoshop, I use photoshop cs2 for the free version? Thank you very much for your attention.
There is no free version of CS2. There is only a special version of CS2 available for those who have bought and need to reinstall it.
-
I got a license number for Adobe Master Collection from my school (Stevens Institute of Technology) and I can't understand how to use it to obtain the product. I created a user name and this is as far as I'm away. I need to download the product and I may not know where to start.
Ask someone to your school... You have a Cloud redemption code, or a number of series CS6?
If cloud, http://helpx.adobe.com/x-productkb/global/redemption-code-help.html Redemption Code and
http://www.Adobe.com/products/creativecloud/FAQ.html
http://helpx.adobe.com/creative-cloud/help/install-apps.html to install or uninstall
http://forums.Adobe.com/community/download_install_setup/creative_cloud_faq
What is http://helpx.adobe.com/creative-cloud/help/creative-cloud-desktop.html
To start https://helpx.adobe.com/creative-cloud.html the Cloud
If CS6, download to other downloads and enter your serial number when prompted
Once again, your 1 resource is your school
-
How to use JavaScript to change the named view
How to use JavaScript to change the named view
Do you mean to jump to a specific Destination named within a PDF file? SE so there
the method gotoNamedDest of the Document object.
-
How to use CSS to create the horizontal navigation bar in Dreamweaver CS6
How to use CSS to create the horizontal navigation bar in Dreamweaver CS6
One of the ways to do this is the following: Editor Tryit v1.9
You can also use floats to get something in the same direction.
-
How to use JavaScript to limit the number of boxes to check the user can select a PDF form?
How to use JavaScript to limit the number of boxes to check the user can select a PDF form?
Here's the working solution
Please rename all the boxes like chkBox0, chkBox1... .chkBox30 (must start with 0)
Value of exports of each checkbox must be "Yes" (default value is Yes)
Place this Code at the level of the Javascript Document
-Code JavaScript document-
var counter = 0; Checked the meter
-Count boxes checked again when the document is closed and reopened.
for (var i = 0; i)<= 30;="">=>
If (getField("chkBox"_+_i).value == 'Yes') {}
Counter += 1;
}
}
-The function of validation-
function validateCheckBox(name,value) {}
If (value == 'Yes' & meter)<>
Counter += 1;
} else if (value == 'Off') {}
counter = 1;
} else {}
getField ("name") .value = "Off";
App.Alert ("you can select only four checkboxes");
}
}
-End of Document Javascript-
Now put this line in the MouseUp event all checkboxes
Box - Code MouseUp event.
validateCheckBox (event.target.name, event.target.value);
//----------------------------------------------------------------------------------
Hope this will help
Kind regards
Post edited by: Mohammad Irfan
-
If im playing Solitaire on my PC, and then walk for a few minutes, I always connect when I get back. He's just going too fast blocking mode.
I would change the amount of time it takes to switch to lock mode.
If im playing Solitaire on my PC, and then walk for a few minutes, I always connect when I get back. He's just going too fast blocking mode.
I would change the amount of time it takes to switch to lock mode.
Hello
Check the settings of the screen saver.
Click on the desktop and select personalize. At the bottom of the window, click the screen saver icon.
Make sure that the summary display the logon screen option is not selected. If this is the case, Remove the check in this option box and select Apply/OK.
Even if a screen saver is not enabled, this option of recovery will force the system to switch to the screen to logon to the selected time interval.
Tell us what you find.
Concerning
-
How to use photoshop to edit my Web site graphics?
I have a Web site here: http://www.christmascountdown.org How to export the graphic on my site to modify the graphics using photoshop and what format is in when you edit and then when load on the site? Thank you.
If its your website, you need to know. If someone built it for you you can look at the page source in your browser to see what they were doing. Use of website .jpg .png .gif and .ico mainly for example it is a JPEG on this site. This is a link to this site.
-
How to use Photoshop without an internet connection
I need to use Photoshop, but due to problems of security of the program is not allowed to connect to the Internet. What are the versions that I can use and how to get them? 99 days/30 days is not the solution (with CC).
You can always download CS6 on another computer and transfer to your safe to install computer. You must activate it if.
Does that help?
Successfully Setup & activation CS6 (not creative cloud) behind an authenticating Proxy Server
Without knowing more details, we can only guess. Also I'm not sure if it is possible to use CC without ever connecting to the Internet.
Maybe you are looking for
-
So I have all my contacts linked to their facebook of google and yahoo, but now the phone seems to be forcing me to use their full names rather than the names of nick. I would like to know the name of MOM or dad pop up instead of first names of my pa
-
How do I run the .srt files in wmp 12
original title: How do I run the .srt files this subtitle files in wmp 12 s? I downloaded directvobsub... but it isn't working... I bought a sony vaio e series laptop computer... I downloaded movies to watch and I'm interested to watch in vlc or othe
-
I get the error code 80070005 when you try to download updates 'KB975364' and "KB972145" _
I can't get these important updates. I tried for more than a week to get them, and no new updates have not developed since then. No solutions are available in the help and support center or online.
-
How to get floating apps in addition to homescreen of10.1 tablet?
How to remove floating apps from home screen of Tablet 10.1 AND how do you remove to destinations of cards when you won't need them once again? This tablet is cruelly a manual and instructions for those of us who aren't tech geeks!
-
BlackBerry Smartphones international travel - England
I was in England at Christmas and I could send and receive texts (no problem), I could receive emails fine, but when I tried to send an email, she would not cross. Icon next to him was a clock and it just sat there and never sent. I thought they wo